Association of tumor and histocompatibility antigens in sera of lymphoma-bearing mice.

Soluble tumor-associated antigens and histocompatibility antigens were detected in sera of A/J mice bearing a spontaneous lymphoma (Ll l l7) by the inhibition of the cytotoxicity of rabbit anti-Ll l l7 serum and of alloantisera to normal A/J antigens, respectively; the rabbit antiserum was rendered specific for lymphoma antigens by extensive absorption with normal A/J cells and alloantisera were produced in C57BL/6J and C3H mice. Both types of antigen were isolated together from serum of tumor-bearing animals, with reverse immunosorbents prepared by insolubilization of either rabbit anti-Lll l7 antibodies or of the anti-A/J alloantibodies, and were also present in the supernatant of the medium used for culturing L l l l 7 cells. It is suggested that the determinants responsible for these two antigenic specificities were associated with the same molecular moiety.